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pcdna3 1 s2  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcdna3 1 s2
    Pcdna3 1 S2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+s2/SUCROSE+EP%2FBP%2FNF+12KG/pm41722661-102-5-25
    Average 99 stars, based on 1 article reviews
    pcdna3 1 s2 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Construct:

    Article Title: Identification of three conserved linear B cell epitopes on the SARS-CoV-2 spike protein
    Article Snippet: The HEK293T cells were cultured in a 12-well plate in a DMEM medium (Gibco, Cat No. A4192101, USA) supplement with 8% FBS. .. The constructed recombinant plasmid pcDNA3.1-NTD, pcDNA3.1-RBD, and pcDNA3.1-S2 were transfected into 293T cells by LipofectamineTM 2000 (Invitrogen, Cat. No. 11668027, USA) in which the cell density reached 70–80%. ..

    Article Title: Identification of three conserved linear B cell epitopes on the SARS-CoV-2 spike protein.
    Article Snippet: The HEK293T cells were cultured in a 12-well plate in a DMEMmedium (Gibco, Cat No. A4192101, USA) supplement with 8% FBS. .. The constructed recombinant plasmid pcDNA3.1-NTD, pcDNA3.1-RBD, and pcDNA3.1-S2 were transfected into 293T cells by LipofectamineTM 2000 (Invitrogen, Cat. No. 11668027, USA) in which the cell density reached 70–80%. ..

    Recombinant:

    Article Title: Identification of three conserved linear B cell epitopes on the SARS-CoV-2 spike protein
    Article Snippet: The HEK293T cells were cultured in a 12-well plate in a DMEM medium (Gibco, Cat No. A4192101, USA) supplement with 8% FBS. .. The constructed recombinant plasmid pcDNA3.1-NTD, pcDNA3.1-RBD, and pcDNA3.1-S2 were transfected into 293T cells by LipofectamineTM 2000 (Invitrogen, Cat. No. 11668027, USA) in which the cell density reached 70–80%. ..

    Article Title: Identification of three conserved linear B cell epitopes on the SARS-CoV-2 spike protein.
    Article Snippet: The HEK293T cells were cultured in a 12-well plate in a DMEMmedium (Gibco, Cat No. A4192101, USA) supplement with 8% FBS. .. The constructed recombinant plasmid pcDNA3.1-NTD, pcDNA3.1-RBD, and pcDNA3.1-S2 were transfected into 293T cells by LipofectamineTM 2000 (Invitrogen, Cat. No. 11668027, USA) in which the cell density reached 70–80%. ..

    Plasmid Preparation:

    Article Title: Identification of three conserved linear B cell epitopes on the SARS-CoV-2 spike protein
    Article Snippet: The HEK293T cells were cultured in a 12-well plate in a DMEM medium (Gibco, Cat No. A4192101, USA) supplement with 8% FBS. .. The constructed recombinant plasmid pcDNA3.1-NTD, pcDNA3.1-RBD, and pcDNA3.1-S2 were transfected into 293T cells by LipofectamineTM 2000 (Invitrogen, Cat. No. 11668027, USA) in which the cell density reached 70–80%. ..

    Article Title: Identification of three conserved linear B cell epitopes on the SARS-CoV-2 spike protein.
    Article Snippet: The HEK293T cells were cultured in a 12-well plate in a DMEMmedium (Gibco, Cat No. A4192101, USA) supplement with 8% FBS. .. The constructed recombinant plasmid pcDNA3.1-NTD, pcDNA3.1-RBD, and pcDNA3.1-S2 were transfected into 293T cells by LipofectamineTM 2000 (Invitrogen, Cat. No. 11668027, USA) in which the cell density reached 70–80%. ..

    Transfection:

    Article Title: Identification of three conserved linear B cell epitopes on the SARS-CoV-2 spike protein
    Article Snippet: The HEK293T cells were cultured in a 12-well plate in a DMEM medium (Gibco, Cat No. A4192101, USA) supplement with 8% FBS. .. The constructed recombinant plasmid pcDNA3.1-NTD, pcDNA3.1-RBD, and pcDNA3.1-S2 were transfected into 293T cells by LipofectamineTM 2000 (Invitrogen, Cat. No. 11668027, USA) in which the cell density reached 70–80%. ..

    Article Title: Identification of three conserved linear B cell epitopes on the SARS-CoV-2 spike protein.
    Article Snippet: The HEK293T cells were cultured in a 12-well plate in a DMEMmedium (Gibco, Cat No. A4192101, USA) supplement with 8% FBS. .. The constructed recombinant plasmid pcDNA3.1-NTD, pcDNA3.1-RBD, and pcDNA3.1-S2 were transfected into 293T cells by LipofectamineTM 2000 (Invitrogen, Cat. No. 11668027, USA) in which the cell density reached 70–80%. ..

    Synthesized:

    Article Title: Optineurin enhances the chemoresistance by activating mitophagy in acute myeloid leukemia with NPM1 mutation.
    Article Snippet: Acute myeloid leukemia (AML) with nucleophosmin 1 (NPM1) mutation is a common genetic subtype with unique pathological features.. However, current therapies remain challenged by relapse and drug resistance, underscoring an urgent need for novel therapeutic strategies.. In this study, we identified hyperactivated mitophagy as a critical metabolic vulnerability in NPM1-mutated AML through bioinformatics analysis and experimental validation.



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